[Immunology] RE: Immuno Digest, Vol 15, Issue 5

J. Diaka via immuno%40net.bio.net (by jdiaka from fau.edu)
Wed Oct 11 13:50:19 EST 2006


I am about to do some work on human prostate and breast cancer cells with
maspin; and I am wondering if there is some one out there who will be
willing to give me a vial of recombinant maspin. I will be very grateful.
I am desperate and I need help.


James K Diaka DVM PhD.



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Subject: Immuno Digest, Vol 15, Issue 5

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Today's Topics:

   1. Re: Anti-CD20 monoclonal antibody for use in BALB/c (Focosi)


----------------------------------------------------------------------

Message: 1
Date: 10 Oct 2006 13:37:45 -0700
From: "Focosi" <mi from interhealth.info>
Subject: [Immunology] Re: Anti-CD20 monoclonal antibody for use in
	BALB/c
To: immuno from net.bio.net
Message-ID: <1160512665.630897.129430 from k70g2000cwa.googlegroups.com>
Content-Type: text/plain; charset="iso-8859-1"

Dear Christian,
I needed a mouse anti-mouse CD20 mAb for intraperitoneal injection into
BALB/c mice. I just found in PubMed what I was looking for, i.e. MB20-1
mAb (see http://intimm.oxfordjournals.org/cgi/content/full/16/1/119).
Hope this could help someone else facing my same hurdle.
Thanks

Christian Praetorius ha scritto:

> "Focosi" <mi from interhealth.info> wrote:
>
> >I'm just looking for a murine anti-CD20 mAb to be used against a BALB/c
>
> You can take any anti-CD20 AB that recognizes mouse CD20. It doesn't
> make a difference if you take BALB/C or Rosa26 mice, e.g.
>
> >B-cell lymphoma line expressing CD20. I would prefer to look at a
> >reference to a peer-reviewed paper to get a reference schedule and
> >route of administration, but anyway any suggestion based on personal
> >experience is very welcome !
>
> The main question is still not answered: What do you want to do?
> Western blot? Immunohistochemistry? Flow cytometry? For each of them,
> you will need different labelled antibodies, so thats the first
> question. And if you will answer this questions, you can also have a
> look into the Pubmed using your topics for the search.
> 
> Christian
> 
> -- 
> X-no-Sig: yes



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