Allison:
Funny you'd ask. I'm watching that very thing with my P3X based fusions. Gentle pipetting or a smack on the side of the flask will dislodge most. I guess they are just sticky after they have been fused. However, I can't speak to the viability issue you bring up. The one's in the media are alive and I increase the numbers by getting more dislodged from the plastic.
David
-----Original Message-----
From: immuno-bounces from oat.bio.indiana.edu on behalf of Allison
Sent: Thu 10/12/2006 2:09 PM
To: immuno from magpie.bio.indiana.edu
Subject: [Immunology] adherent cells after fusion
Any one have ideas on why sometimes I get adherent cells growing after
doing a standard splenocyte - Sp2/0 fusion with PEG? This last time I
had very few hybridoma clones, lots of dead/clumped cells, and afair
number of adherent cells.
Thanks
Allison
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