[Immunology] adherent cells after fusion

Haviland, David L via immuno%40net.bio.net (by David.L.Haviland from uth.tmc.edu)
Thu Oct 12 22:45:31 EST 2006


Funny you'd ask.   I'm watching that very thing with my P3X based fusions.   Gentle pipetting or a smack on the side of the flask will dislodge most.   I guess they are just sticky after they have been fused.  However, I can't speak to the viability issue you bring up.  The one's in the media are alive and I increase the numbers by getting more dislodged from the plastic. 


-----Original Message-----
From: immuno-bounces from oat.bio.indiana.edu on behalf of Allison
Sent: Thu 10/12/2006 2:09 PM
To: immuno from magpie.bio.indiana.edu
Subject: [Immunology] adherent cells after fusion
Any one have ideas on why sometimes I get adherent cells growing after 
doing a standard splenocyte - Sp2/0 fusion with PEG?  This last time I 
had very few hybridoma clones, lots of dead/clumped cells, and afair 
number of adherent cells.


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