[Immunology] adherent cells after fusion

Allison via immuno%40net.bio.net (by allison from nospam.com)
Fri Oct 13 10:41:05 EST 2006

The adherent cells look spread out, as if they might be fibroblasts. 
Not the kind of thing that can be dislodged by gentle pipetting (this I 
already see with my Sp2/0 cells and I would consider it to be normal).


Haviland, David L wrote:

> Allison:
> Funny you'd ask.   I'm watching that very thing with my P3X based fusions.   Gentle pipetting or a smack on the side of the flask will dislodge most.   I guess they are just sticky after they have been fused.  However, I can't speak to the viability issue you bring up.  The one's in the media are alive and I increase the numbers by getting more dislodged from the plastic. 
> David
> -----Original Message-----
> From: immuno-bounces from oat.bio.indiana.edu on behalf of Allison
> Sent: Thu 10/12/2006 2:09 PM
> To: immuno from magpie.bio.indiana.edu
> Subject: [Immunology] adherent cells after fusion
> Any one have ideas on why sometimes I get adherent cells growing after 
> doing a standard splenocyte - Sp2/0 fusion with PEG?  This last time I 
> had very few hybridoma clones, lots of dead/clumped cells, and afair 
> number of adherent cells.
> Thanks
> Allison
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