The adherent cells look spread out, as if they might be fibroblasts.
Not the kind of thing that can be dislodged by gentle pipetting (this I
already see with my Sp2/0 cells and I would consider it to be normal).
Allison
Haviland, David L wrote:
> Allison:
>> Funny you'd ask. I'm watching that very thing with my P3X based fusions. Gentle pipetting or a smack on the side of the flask will dislodge most. I guess they are just sticky after they have been fused. However, I can't speak to the viability issue you bring up. The one's in the media are alive and I increase the numbers by getting more dislodged from the plastic.
>> David
>> -----Original Message-----
> From: immuno-bounces from oat.bio.indiana.edu on behalf of Allison
> Sent: Thu 10/12/2006 2:09 PM
> To: immuno from magpie.bio.indiana.edu> Subject: [Immunology] adherent cells after fusion
>> Any one have ideas on why sometimes I get adherent cells growing after
> doing a standard splenocyte - Sp2/0 fusion with PEG? This last time I
> had very few hybridoma clones, lots of dead/clumped cells, and afair
> number of adherent cells.
>> Thanks
>> Allison
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