[Immunology] RE: Immuno Digest, Vol 15, Issue 8

[Hardaway, John Crawford]

Fibroblasts are not good when culturing fused cells.  They often will
dominate the culture and utilize media nutrients that your fused cells
need.  Often presence of fibroblasts occurs when a substantially aged
animal was used as the splenocyte donor.  To circumvent this, I've kept
the age of my immunized animals low prior to fusion.
-John 

-----Original Message-----
From: immuno-bounces from oat.bio.indiana.edu
[mailto:immuno-bounces from oat.bio.indiana.edu] On Behalf Of
immuno-request from oat.bio.indiana.edu
Sent: Saturday, October 14, 2006 12:02 PM
To: immuno from magpie.bio.indiana.edu
Subject: Immuno Digest, Vol 15, Issue 8

Send Immuno mailing list submissions to
	immuno from net.bio.net

To subscribe or unsubscribe via the World Wide Web, visit
	http://www.bio.net/biomail/listinfo/immuno
or, via email, send a message with subject or body 'help' to
	immuno-request from net.bio.net

You can reach the person managing the list at
	immuno-owner from net.bio.net

When replying, please edit your Subject line so it is more specific than
"Re: Contents of Immuno digest..."


Today's Topics:

   1. Re: adherent cells after fusion (Allison)


----------------------------------------------------------------------

Message: 1
Date: Fri, 13 Oct 2006 11:41:05 -0400
From: Allison <allison from nospam.com>
Subject: Re: [Immunology] adherent cells after fusion
To: immuno from net.bio.net
Message-ID: <BuKdne-W0_QPLrLYnZ2dnUVZ_t2dnZ2d from mcgill.ca>
Content-Type: text/plain; charset=us-ascii; format=flowed

The adherent cells look spread out, as if they might be fibroblasts. 
Not the kind of thing that can be dislodged by gentle pipetting (this I
already see with my Sp2/0 cells and I would consider it to be normal).

Allison


Haviland, David L wrote:

> Allison:
> 
> Funny you'd ask.   I'm watching that very thing with my P3X based
fusions.   Gentle pipetting or a smack on the side of the flask will
dislodge most.   I guess they are just sticky after they have been
fused.  However, I can't speak to the viability issue you bring up.  The
one's in the media are alive and I increase the numbers by getting more
dislodged from the plastic. 
> 
> David
> 
> -----Original Message-----
> From: immuno-bounces from oat.bio.indiana.edu on behalf of Allison
> Sent: Thu 10/12/2006 2:09 PM
> To: immuno from magpie.bio.indiana.edu
> Subject: [Immunology] adherent cells after fusion
>  
> Any one have ideas on why sometimes I get adherent cells growing after

> doing a standard splenocyte - Sp2/0 fusion with PEG?  This last time I

> had very few hybridoma clones, lots of dead/clumped cells, and afair 
> number of adherent cells.
> 
> Thanks
> 
> Allison
> _______________________________________________
> Immuno mailing list
> Immuno from net.bio.net
> http://www.bio.net/biomail/listinfo/immuno
> 
> 


------------------------------

_______________________________________________
Immuno mailing list
Immuno from net.bio.net
http://www.bio.net/biomail/listinfo/immuno

End of Immuno Digest, Vol 15, Issue 8
*************************************