[Immunology] Mouse anti-human CD9 and non-fluorescent double immunocytochemistry staining

Ho Kian Kheong via immuno%40net.bio.net (by kiankheong from gmail.com)
Mon Oct 23 10:17:32 EST 2006


Hi folks!

I have to 2 questions:

1) I am looking for mouse anti-human anti-CD9 antibodies. Apparently, 
Dako and Chemicon have discontinued their range and I am in desperate 
need of this antibody in order to do negative-selection immunopurification.

2) Has anyone had good encounter in working with non-fluorescent double 
immunocytochemistry staining? I am planning to carry out staining on two 
types of cytoplasmic filaments: vimentin and cytokeratin-7. Any advice 
will be greatly appreciated.

Thanks a lot.

Cheers,

Ho Kian Kheong



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