[Immunology] T cell isolation

Soamaeshwar SHARMA via immuno%40net.bio.net (by u0422934 from uel.ac.uk)
Wed Oct 25 10:47:48 EST 2006



Hi All

           Hope you might be able to help me out with my work. I need to find
a successful protocol with materials stating how to using ficoll gradient to
isolate t cells from peripheral human blood.

	Beyond that a protocol to expand t cell numbers with PHA before
introducing superantigen to them 

	I am beginning to realise this is quite a demanding competitive area
or so I think, are there any useful journals web sites you may be able to
recommend
.


I have come across using a nylon column but am not sure what this invloves. I
would apreciate any experienced opinions such opting for using sheep red
blood cell mixtures to fix the t cells.



Thank you very much



 soamaesh.sharma from ntlworld.com


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