IUBio

[Immunology] Re: Immuno Digest, Vol 14, Issue 14

lit lei cheng linalit at yahoo.com
Tue Sep 26 23:14:17 EST 2006


Dear Dr Kumi-Diaka,
   
  I bought the K562 from ATCC and revived the frozen cell in IMDM with 10% FCS. Then I changed the medium to DMEM with 10% FCS. It also grow well in RPMI 1640 with 10% FCS.
   
  Regards,
  Lit Lei Cheng
  Dep of Physiology 
  Faculty of Medicine
  University of Malaya
   
  
immuno-request at oat.bio.indiana.edu wrote:
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Today's Topics:

1. RE: Immuno Digest, Vol 14, Issue 12 (J. Diaka)


----------------------------------------------------------------------

Message: 1
Date: Mon, 25 Sep 2006 16:17:54 -0400
From: "J. Diaka" 
Subject: [Immunology] RE: Immuno Digest, Vol 14, Issue 12
To: immuno at magpie.bio.indiana.edu
Message-ID: <000501c6e0df$b5453ba0$392a5b83 at irm.ad.fau.edu>
Content-Type: text/plain; charset="us-ascii"


Hi all,

I am wondering if someone could give some hints as to what media etc I need
to successfully culture K56 cells. I have tried DMEM, and other standard
complete media to grow these cells without any success. I need help.

Thanks,


Kumi-Diaka

jdiaka at fau.edu


-----Original Message-----
From: immuno-bounces at oat.bio.indiana.edu
[mailto:immuno-bounces at oat.bio.indiana.edu] On Behalf Of
immuno-request at oat.bio.indiana.edu
Sent: Thursday, September 21, 2006 1:03 PM
To: immuno at magpie.bio.indiana.edu
Subject: Immuno Digest, Vol 14, Issue 12

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Today's Topics:

1. YAC-1 cells (Ri-Yao Yang)


----------------------------------------------------------------------

Message: 1
Date: Wed, 20 Sep 2006 09:30:52 -0700
From: Ri-Yao Yang 
Subject: [Immunology] YAC-1 cells
To: immuno at magpie.bio.indiana.edu
Message-ID: <80EC28B5-8BC1-4862-9572-BD2B3548AE5F at ucdavis.edu>
Content-Type: text/plain; charset=US-ASCII; delsp=yes;
format=flowed

Hi,

I need the YAC-1 cell line for the cytotoxicity assay of NK cells. I 
wonder if someone has this cell line and is willing to send me an 
aliquot.

Thanks,

Ri-Yao Yang, Ph.D.
Assistant Immunologist
Department of Dermatology
University of California, Davis
School of Medicine
Research III, 3100C
4645 Second Avenue
Sacramento, CA 95817

Phone: (916) 734-8504
Fax: (916) 734-8386
Email:ryyang at ucdavis.edu






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