[Immunology] flow cytometry prep solution

Lectin via immuno%40net.bio.net (by lectin At gmail.com)
Thu Feb 1 03:49:05 EST 2007


For flow cytometric immunophenotyping:

Could someone help me with the exact contents of the TQ-prep (BC) solutions
for preparation of whole blood for antibody staining (want to prepare these
in-house)?

Or, does anyone use a well-standardized in-house FCM phenotyping preparation
solution-s?

For fixation of cell bound antigen-antibody complexes (again for FCM), which
concentration of paraformadehyde works better, 1 or 2%, or ?

And last, some dissolve paraformadehyde in dH2O, while others use PBS. Which
one is more suitable for immunophenotyping of PBMCs?


Thanks in advance for any comment...


lectin


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