(by anto.ilmago from gmail.com)
Thu Feb 21 11:49:21 EST 2008
I've recently tried a PE-conjugated anti human PD-1 antibody, to stain
some human PBMCs in inflammatory and autoimmune conditions.
Some published papers use a FITC-conjugated antibody, same clone as
mine (MIH4), but the graphs for their staining show an increased
signal compared to mine.
Could it be possible that the PE conjugation affects the binding of
the antibody, reducing its specificity compared to FITC?
Waiting for some replies...
Thanks in advance :-)
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