[Immunology] ELISA: blanking and data interpretation question
(by tdenysse from bccrc.ca)
Tue Feb 26 18:05:41 EST 2008
I have a question on how to blank the results from a typical double
sandwich ELISA assay. The assay measures therapeutic antibody in
human serum and I have two blanks available : assay buffer and
calibrator "Zero" containing 0.05% of human serum (after MRD 1:2000)
and no analyte.
So far I was blanking all results only with the assay buffer value. Is
this totally incorrect? I was assuming that calibrator "Zero" should be
part of the calibration curve.
If standard "Zero" should be used as a blank, then how to construct
the standard curve in the 4PL model? It would mean that standard zero is
not included in the model. Would this be a correct approach?
Thank you for your help in this educational exercise
Investigational Drug Program
BC Cancer Agency
phone: 604-675-8000, ext 7026
e-mail: tdenysse from bccrc.ca
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