[Immunology] ELISA protocol

Carlos Duarte Cano via immuno%40net.bio.net (by carlos.duarte from cigb.edu.cu)
Fri Apr 17 16:50:17 EST 2009

Dear Dr Sudheesh

You may use both but the second one is better since some inespecific
stimulation can slightly increase the background in the ELISA. The best
is to have a group of sera and to use the mean  and the STD to calculate
the cut off.


Carlos Duarte

-----Original Message-----
From: immuno-bounces from oat.bio.indiana.edu
[mailto:immuno-bounces from oat.bio.indiana.edu] On Behalf Of m s sudheesh
Sent: Friday, April 17, 2009 1:27 PM
To: immuno from magpie.bio.indiana.edu
Subject: [Immunology] ELISA protocol

Dear All,
  I am working on the development of nanoparticulate vaccine. While
performing ELISA potocol the end point titre was expressed as the
logarithm of the reciprocal of the last dilution which gave an optical
density at 490nm above the optical density of negative control. What
should be the negative control is it preimmune sera or the sera of
animals trated with blank nanoparticles or the well containing only
substrate. Kindly help me.

M.S.Sudheesh,Research Fellow,
Pharm Biotech Div, Department of Pharmaceutical Sciences, Dr. H.S.Gour
University, Sagar, M.P,India,470001 Phone +91-9826415402
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