[Immunology] bacterias 41(DE3) e C43(DE3)

Itabajara Vaz - UFRGS via immuno%40net.bio.net (by itabajara.vaz from ufrgs.br)
Mon Apr 20 17:12:05 EST 2009


I am needing the C41(DE3), C41(DE3)pLysS and C43(DE3), C43(DE3)pLysS (E. 
coli bacterias)
Does anybody have these bacterias?
//Can anybody help me?
Thank you very much,

**C41(DE3), C41(DE3)pLysS and C43(DE3), C43(DE3)pLysS
E. coli BL21(DE3) strains, like Lucigen's E. cloni EXPRESS Competent 
Cells provide reliable expression of many proteins cloned into T7 
expression vectors (e.g., pET or Lucigen's pSMART®-cDNA vectors). 
However, in some cases expression is minimal or not detectable because 
the recombinant protein, when expressed, is deleterious or lethal to 
these standard BL21 strains. Examples of such toxic proteins include 
many membrane proteins, some cytoplasmic proteins, and nucleases. 
Unfortunately, successful expression of one or more toxic proteins is 
often important to the experimental goal.



Itabajara da Silva Vaz Junior
Centro de Biotecnologia  - UFRGS
C.P. 15005
Av. Bento Goncalves 9500
Predio 43421 - Campus do Vale
91501-970  Porto Alegre RS   Brasil
Fone (51) 33086078    Fax (51)3308-73-09  [00 55 51 3308 7309]

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