[Immunology] CD56CD16 bright, CD56CD16 dim

David O'Brien via immuno%40net.bio.net (by dobrien001 from rochester.rr.com)
Thu May 14 21:06:01 EST 2009


We have a client who wishes to do a dot plot with CD56PE CD16PE on the y
axis vs CD3 on x axis.  The client wants to separate CD56CD16 bright cells
from CD56CD16 dim cells.  We have run some normal patients and found that
only one population of cells shows up.  There is no clear delineation
between a bright and dim population for CD56CD16 staining.  Has anyone done
this before and does anyone know how to set the gate so that one could
delineate bright and dim cells?



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