[Immunology] cell surface markers for THP-1 cells

Chu, Calvin via immuno%40net.bio.net (by CChu from med.miami.edu)
Thu Sep 24 10:35:39 EST 2009


Hi,

I am currently working with THP-1 cells (ATCC TIB 202).  I mainly work with this cells without differentiating them.  I want to do some cell surface expression analysis of these cells using Flow Cytometry, and I am trying to find some good positive controls to perform the necessary compensation in flow cytometry.

Most of the literature I have found on flow cytometry conducted on THP-1 cells is performed on THP-1 cells which have been differentiated with either PMA or Vitamin D.  And this differentiation causes a tremendous difference in the expression profile of THP-1 cells, especially CD14 which is not expressed in undifferentiated THP-1 cells.

I am wondering whether anybody can suggest a good marker for undifferentiated THP-1 cells.

Thanks.

Calvin.



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