[Immunology] Can 3H thymidine labeling in lymphocytes be
successfully measured after cells are fixed??
geoffreycole from verizon.net
(by geoffreycole from verizon.net)
Thu Oct 20 22:20:46 EST 2011
Is it feasible to chemical fix(e.g.with 1-2% PFA) 3H thymidine pulse
labeled lymphocytes in serum supplemented culture medium and
subsequently harvest them on a cell harvester? While no doubt this
sound methodologically unorthodox and improbable to work, there is a
rationale. Ideally, I would like to pulse label stimulated PBMC and
lightly fix them to biologically inactivate any potential viral agents
present prior their processing on a Tomtec cell harvester. I have
facilities available to me for performing this work under appropriate
biological containment. I am not seriously contemplating this as an
assay procedure but, I was curious if anyone had ever explored this.
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