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Hi,
<p>an alternatine technique for the Cell-ELISA is the cell-membrane-ELISA,
which is described in detail in <font face="Arial,Helvetica">Tur
et al., 2001, Selection of scFv phages on intact cells under low pH conditions
leads to a significant loss of insert-free phages, BioTechniques, 30, 404-408</font>.
It works very well.
<p>Mehmet
<p>yangxx schrieb:
<blockquote TYPE=CITE>is there anyone doing the cell-elisa?if someone did
it ,can you giceme the
<br>protocol.and i have some questions:
<br> 1)if A450 of negative control( using the irrelevance cells)
is high,how
<br>can i reduce this.
<br>2)if i use the cell-culture plate but not the plate that is special
for the
<br>cell-elisa to do this expriment,is there any influence to
the results?
<br>thanks a lot!
<p><<a href="http://www.biowww.net/forum/read.php?f=3&i=427&t=427">http://www.biowww.net/forum/read.php?f=3&i=427&t=427</a>></blockquote>
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