proposed collaboration

daemon at net.bio.net daemon at net.bio.net
Sat Nov 1 10:29:21 EST 1997


To whom it may concern:

I would like to cooperate to do research with you. The proposed research
programe is enclosed.

Song Yunchun
Professor of Genetics

Song Yunchun
 professor of Genetics
 College of Life Sciences
 Wuhan University
 Wuhan 430072
 P.R.China
E-mail:rennan69 at public.wh.hb.cn
           xuezhibi at public.wh.hb.cn

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=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D

The Laboratory of Plant Molecular Cytogenetics

Work team:

There are one professor, two associated professors,one senior
engineer,six Ph D and four
Ms students in our lab..The head is Prof.Song,Yunchun


Research Interests:

   (1)physical mapping of the important function and economic character
genes inrice,maize and barley using in situ hybridization.
   (2)Comparative physical mapping among rice ,maize and barley using in
situ hybridization.
   (3)Characterization of alien introgression in wide-cross derivatives
and transgenic plants of rice, maize and barley
  (4)Analysis of arrangment and organization of genes in the single
chromosome, for example, chromosome No.4 in rice.
   (5)The programed cell death in plants

Project Discription:

   Up to now,the molecular genetic maps have been developed in some  cereal
crops including rice,maize and barley etc.However,the genetic map can only
show  the relative position and distance between the makers on the
chromosome.In order to  know the real position,distance and relationship
between the markers on the  chromosome,physically mapping them is
indispensable.

   In situ hybridization(ISH)is a powerful tool for physical mapping of
genes.We have improved the ISH technique and mapped many RFLP and cDNA
markers in rice and maize.The results showed that the genetic and physical
maps are different  from eath other.Two markers that covered a short
genetic distance might be  physically distant,and vice versa.The RFLP
markers were not distributed all along the whole length of the
chromosome,for example,the markers of linkage group 11 distributed only  in
the short arm of chromosome 11 in rice.Although the exact reason for the
differences  between the genetic and physical maps is still
unknown,according to the comparisons between  this two kinds of maps we
could obtain much deeper insight about the structure of the genome. Our
follow-up work is to physically map the important function and  economic
character genes including those dictating the resistances to
disease,insect,and  tolerance to low temperature,drought stress and soil
which contains rather high  concentration of salt,alkaline,and acid,etc.in
rice,maize and barley.Now we are developing  the sequential fluorescent in
situ hybridization(FISH)and chromosome G- banding  technique,and have
already obtained some success.In the next step,we will adopt the  technique
and use cDNA or molecular markers closely linked with these genes as probe
to locate  this genes onto the special band regions of a given chromosome.

   The objective is to construct a cytological map including the  important
genes in the cereal crops.This map will offer very important information
for developing the  rice biotechnology and understanding the organizations
and functions of the genomes in rice  ,maize and barley.This also will
closely connect the cereal crop research on the molecular level  with that
on the cytological level and promote the development of plant molecular
cytogenetics. There are many homologous segments of chromosomes among
rice,maize and  barley.To compare their physical maps is an effective way
for analysis of their  homogeneities.The knowledge about the homogeneities
will help us to recognize the  relationship of classification and evolution
among these crops.

    It is still unknown how to organize different kinds of genes oneach
chromosome.To study it deeply will increase the insight about fine
structure of chromosomes.     Developmental biology is a precursory field
in life science.It is  evident that the mechanisms of development could be
revealed by studing the time and space  changes of genome structure and
gene activities.


Vitae and Publication List
    Song Yunchun
    Collegy of Life Sciences
    Wuhan University
    Wuhan, 430072
    The People's Republic of China
    Telephone:7882712-4505
    Fax:(027) 7813833

Education Background:
    1956-1961 Plant Genetics Speciality,
    Biology Department,
    Wuhan University
    (A five year schooling)

Professional Experince:
    1961-1978 Assistant, Wuhan University
    1978-1983 Lecture, Wuhan University
    1983-1989 Associated Professor, Wuhan University
    1989-present time Professor, Wuhan University

Honors:
    Visiting Scholar, San Diego State University, USA, 1983-1984.
    Senior Visiting Scholar, USDA-ARS, University of Missouri, USA, 1992.

Areas of Experties:
    Cytogenetics, and Molecular Cytogenetics.

Membership in Professional Societies:
    Chinese Genetics Association
    Chinese Cell Biology Association
    Genetics Society of Canada

Publications Since 1986:
   Song, Yunchun,  Ratty, F.J. and Laurs R.M. 1986. Trasmissiom elctron
microscope of C-banded skipjack tuna chromosomes. J. Fish Biol. 28:419-424

   Ratty, F.J., Song, Y.C. and Laurs, R.M. 1986. Chromosomal analysis of
albacore, Thunnus alalunga, and yellowfin, Thunnus alabacares and
skipjack,Katsowowonus pelamis, tuna. Fishery Bulletin 84(2):469-476.

   Ward, D.G., Wuster-Hill, Ratty, F.J., Ratty, F.J. and Song, Y.C. 1987.
Comparative cytogenetics of Chinese and Japanese raccoon dogs, Nyctereutes
procyonoides. Cytogenet. Cell Genet. 45:177-186.

   Song, Yunchun 1987. Studies on karyotypes and C-banding patterns of
three fish species in Scombridae. Oceanologia Et Limnologia Sinica
18(4):352-356.

   Song, Yunchun, Liu, Lihua and Taing, Xiaolang 1987. Studies of maize
chromosome G-banding by using of ASG method. Acta Genetica Sinica
14(6):424-427.

   Song, Yunchun and Liu Lihua 1989. Comparisons of G-banding patterns
among different cultivars in maize (Zea mays), Plant Chromosome Research
1987 201-206.

   Ding, Yi and Song Yunchun 1989. Studies on G-banding karyotype in
barley (Hordeum vulgar). Acta Botanica Sinica 31(9):684-688.

   Feng, Guohong and Song, Yunchun 1989. Studies on the association of
nucleolus and NOR chromosome in maize through the modified Giemsa Banding
technique Journal of Wuhan Botanical Research 7(4):303-307.

   Ding, Yi and Song, Yunchun 1989. Studies of chromosome G-banding in
barley (Hordeum vulgare). Plant Chromosome Research 1987 387-390.

   Song, Yunchun and Yu, Xianjue 1989. "General Genetics" Book 1 Wuhan
University Publishing Hourse.

   Song, Yunchun And Yu, Xianjue 1990. "General Genetics" Book 2 Wuhan
University Publishing Hourse.

   Song Yunchun and Liu, Lihua 1990. Studies of chromosome G-banding
patterns in maize. Chinese Journal Of Genetics 17(3):181-189.

   Song, Yunchun, Liu Lide, Li, Zhanlin and Liu, Lihua 1990. Chromosomal
studies of superior seed sources in China fir (Cunninghamia lanceolata)
in "Genetics Research in China."  China Science and  Technology Publishing
Hourse, Peking p. 130-131.

   Tain Xianbing and Song, Yunchun 1990. Studies of the origin of A and B
genome in tetraploid wheat using G-banding techniques.  in "Genetics
Research in China."  China Science and Technology  Publishing Hourse,
Peking p. 89.

   Yao Qing, Song, Yunchun and Liu, Lihua 1990. Studies of chromosome
G-banding in rice (Oryza sativa). Chinese Journal Of Genetics 17(3):165-173.

   Li, Zhanlin and Song, Yunchun 1990. Studies on chromosome G-bands in
Cunninghamia lanceolata. Scientia Silvae Sinica. 26(5):452-456.

   Ding, Yi and Song, Yunchun 1990. Analysis of G-banded karyotypes of
wild and cultivated barley in China. in "Genetics Research in China."
China Science and Technology Publishing
Hourse, Peking p. 131.

    Ding, Yi and Song, Yunchun 1991. Comparisons among G-, C-, N-banded
and silver stained chromosomes in Hordeum vulgare. Chinese Science Bulletin
36(19): 1641-1645.

    Tain Xianbing and Song Yunchun 1991. Studies of G banding and its
mechanisms in rye using AEG method. Journal of Zhongkai Agrotechnical
College 4:51-58.

  Song Yunchun, Zhang Feixiong and Liu Lihua 1992. Analysis of chromosome
G-banding patterns in 8 types of maize. Journal of Wuhan University(Natural
Science Edition) No. 1:105-110.

   Zhang, Feixiong, Song, Yunchun and Liu, Lihua 1992. Comparative
studies between G-banded karyotypes in Zea, Euchlaena and Coix.  Journal of
Wuhan University (Natural Science Edition) No. 1:111-116.

   Li, Zhanlin, Song, Yunchun, Lui, Lide and Liu Lihua 1992. Karyotype
analysis of  8 geographic seed source of Cunninghamia lanceolata. Journal of
Central-South Forestry College 12(1):11-17.

   Song, Yunchun, Liu, Lihua, Ding, Yi, Yao, Qing, Zhang, Feixiong,
Tian, Xianbing, Meng, Li, And Chen Jinghua 1992. Comparisons among
G-banding patterns of several Gramineous crops. Proc. Second Sino-Japenese
Sym posium Pl Chromosome. p. 26.

   Hu,Xiaoge and Song Yunchun 1992. Comparative studies of chromosome bandin=
g
patterns of maize local strains frome two main zones in China.
Proc.Second Sino-Japenese Symposium  Pl Chromosome. p. 85.

   Song, Yunchun, Yao Qing, Ding Yi, Zhang Feixiong, Tain Xianbing and
Liu Lihua 1993.

Analysis of stablity and application for AEG method of plant G-banding.
in "Advances in Life Science", China medico-Pharmaceutical Science and
Technology  Publishing Hourse Peking pp 352-365.

   Y.C.Song,Liu,Lihua and Xu,Musheng 1993. Analysis of nature of G-banding
patterns in maize.in"Advances in Life Science",China medio-Pharmaceutical
Science and Technology Publishing Hourse Peking pp 438-443.

Yao,Qing and Y.C.Song 1993.Identification of trisomics in japonica rice
plants by chromosome G-banding.Acta Genetica Sinica 20(4):285-292.

   Ding,Yi,Y.C.Song and Yu,S.J.1993.Studies of chromosome NOR staining in
wild and cultivated barley.Plants Chromosome Research 1992.pp147-152. Chen
Jinhua and Y.C.Song 1993.Studies on G-banding karyotypes of four Oryza
species.Plant Chromosome Research 1992.pp133-139.

  He,S.J.,Gu,M.G.,Yan,Chenheng and Y.C.Song 1993.Studies on Giemsa
banding in two inbred lines derived from distant hybrid parthenocarpy and
their
parents.Plant Chromosome Research 1992.pp381-386.

   Chen,Jinhua,Y.C.Song,Wang,Mingquan and Song,Wenzhen 1993.Studies on
polymorphisms of G-banded karyotypes in Oryza rufipogon Griff.Acta Botanica
Sinica 35(11):844-846.

   Li,Zhanlin,Y.C.Song,Liu,Lide and Liu,Lihua 1993.Studies on chromosome
G-banging patterns of 8 geographic seed sources,of Cunninghamia
lanceolata.Journal of Central-South Forestry College 13(1):64-68

   Li,Zhanlin,Y.C.Song,Liu,Lide and Liu,Lihua 1994.Studies on chromosome
N-banding of Cunninghamia lanceolata.Journal of Central-South Forestry
College 14(1):40-43

   Y.C.Song and Gustafson,J.P.1993.Physical mapping of 5S rDNA gene
complex in rice(Oryza sativa L.).Genome 36(4):658-661.

Y.C.Song 1994.Comparisons of G-banding patterns in six species of the
Poaceae Hereditas 121:31-38.

   Cao,Jiashu,Cao,Shouchun and Y.C.Song 1994.Studies on Chromosome
G-banding in 10-Chromosome Vegetables of Brassica 21(3):257-263.

   Y.C.Song and Gustafson,J.P.1995.The physical location of fourteen RFLP
markers in rice (Oryza sativa L.).Theor.Appl.Genetics 90:113-119.

He,Changrui,Y.C.Song and
Liu,Lihua 1995 Comparative Studies on G-banded Karyotypes of the three
lines and F1 Hybrids in Sorghum 22(5):400-405.

Yang,taixing,Guo,Lequn,Yan,Chunhong and Y.C.Song 1995.Ploidy Variation of
Chemically-Induced Parthenogenic Maize Plants 27(5):406-412.

   Wu,Shijun,Bian,Pingguan,Huang,Deying,Y.C.Song,Liu,Lihua and Zhao,Min
1995.Effect of Magnetic Field on Karyotype and G-band of Rice Chromosome
Journal of South-Central college for Nationalities(Natural Science
Edition)14(2):6-12.

   Ding,Yi,Y.C.Song and Yu,Xianjue 1995.Cluster Analysis and Genetic
Studies of Esterase Isozyme Zymograms in Barley of China 41(6):729-734.

   Ding,Yi and Y.C.Song 1996.Analysis of G-band Fluctuation and
Correspondence of G-banging Patterns to Chromosome Patterns in Barley.Acta
Genetica Sinica
23(4):268-275.

   Ren,Nan and Y.C.Song 1996.The physical location of the maize cdc2 gene
by in situ hybridization(Digoxigenin labeling).J.Wuhan Univ.(Natural Science
Edition) 42(6).

   Ren Nan and Song Yuncun et al. 1997. The physical location of the
genes cdc2  and   prh1 in maize(Zea mays L.).  Hereditas. (Sweden).126:211-2=
17.

    Y.C.Song,Ren,N,Mao,N,and Liu,L.Simultaneous chromosome G-banding in situ
hybridization of RFLP markers in maize 1996.Genetics Cooperation
Newsletter.Vol 70:70-73.

  Xue-Zhi Bi,Y.C.Song,Nan Ren and Li-Hua Liu, 1997.  Physical Mapping of
CaM and Ca2+-ATPase Genes in Rice by in situ hybridization. Rice Genetics
Newsletter,13:82-85
 Ren,N,Y.C.Song, Physical mapping the genes cdc2 and prh1 in
maize(Zeamays L.) Hereditas, in press

  Ren,N,Y.C.Song, Comparative mapping the Ca-ATPase gene in the rice and
maize(Submitted).  Physical location the gene cdc2 and the gene of
cyclin=A2=DB(B-like)in maize(Zea mays L.)(Digoxigenin and Biotin
labeling)(Submitted).











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