We need to isolate DNA (for PCR) from many thousands of maize plants.
We've found some very quick/easy extraction protocols that work on fresh
tissues. However, the big stumbling block is tissue grinding. We would
like to avoid the necessity of freeze-drying the tissue prior to
extraction, but so far we haven't had good luck grinding fresh tissue in a
Has anyone worked out a reliable, easy protocol from fresh tissue?
Patrick S. Schnable
Professor of Plant Genetics
G405 Agronomy Hall
Iowa State University
Ames, IA 50011 USA
schnable at iastate.edu