high-throughput DNA extraction

Patrick S Schnable schnable at iastate.edu
Wed Nov 25 09:29:22 EST 1998


We need to isolate DNA (for PCR) from many thousands of maize plants.
We've found some very quick/easy extraction protocols that work on fresh
tissues.  However, the big stumbling block is tissue grinding.  We would
like to avoid the necessity of freeze-drying the tissue prior to
extraction, but so far we haven't had good luck grinding fresh tissue in a
paint shaker.

Has anyone worked out a reliable, easy protocol from fresh tissue?

Pat



Patrick S. Schnable
Professor of Plant Genetics
G405 Agronomy Hall
Iowa State University
Ames, IA  50011 USA
schnable at iastate.edu
515-294-0975 (office)
515-294-2299 (fax)
http://www.public.iastate.edu/~schnable/




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