Maize Root RNA Extraction

[Steve Haines]

Dear All,

I am looking for a reliable protocol for extracting total RNA from maize
root tissue. I want as high a yield as possible. I currently use an SDS
based buffer with several phenol/chloroform treatments but I seem to get
a very low yield (in some cases no yield at all!!).

I hope you can help...

Many Thanks

Steve Haines
Crop Genetics Group
IACR-Long Ashton Research Station
Bristol BS41 9AF
E-mail : stephen.haines at bbsrc.ac.uk