Maize Genetics Research Needs
Michael D. McMullen
mcmullen at teosinte.agron.missouri.edu
Sun Apr 2 09:45:15 EST 2000
Comprehensive arrayed cDNA libraries, implies comprehensive cDNA resources,
maybe a cart-before-the-horse issue.
1, 2, 3, 4, 10, 14, 16 all need done, priority should be set by which
technologies are ready and cost-effective, not wishful thinking.
I don't see the point of #15 (one or more very large populations), IBM will
give the resolution of 900 individuals at the operating cost of 300. If you
are going to fine-map it is in a population segregating for your trait of
Is #13 (SSRs) more about marketing and distribution then about development?
Ed Coe wrote:
> To Maize Netters:
> Before, during, and after the 1999 Maize Genetics Meeting in
> Wisconsin, and at the Maize Forum 2000 in San Diego, an ad
> hoc Maize Committee has requested and received information
> about resource deficiencies that are limiting the
> productivity of maize genetics research in the public
> sector. Our results indicate the following major needs, in
> these approximate priority orders:
> 1. Improved maize transformation capacity in the public
> sector [for your information, an action plan is being
> undertaken to bear this need to the Interagency Working
> Group for Plant Genomics].
> 2. Better expression profiling tools.
> 3. Comprehensive arrayed cDNA libraries.
> 4. Tissue specific and developmentally controllable
> 5. More community building activities, particularly for
> attracting new scientists to maize genetics.
> 6. Less emphasis on megagrants, more on individual
> investigator awards.
> 7. Improvements in the user-friendliness of MaizeDB.
> 8. Better coordination of tool development for the community.
> 9. Improved comparative data and bioinformatics, for the
> grasses and between maize mapping populations.
> 10. Fully accessible, appropriately redundant enhancer
> traps, promoter traps, and knockouts systems in the public
> sector for maize reverse genetics.
> 11. Improved reverse genetics tools in other grass species
> (e.g., rice) to allow comparisons to orthologous maize mutants.
> 12. More complete and more accessible storage and
> distribution of maize EST data and clones.
> 13. More maize SSRs available inexpensively to all.
> 14. Large mutagenized (small deletions, EMS, transposon
> tagged) populations, in an inbred (B73) background.
> 15. One or more very large (>2000 progeny) populations
> mapped with all of the available RFLP, SSR, etc. markers
> that can then be used for fine structure mapping by all
> maize researchers.
> 16. Proteome knowledge and research, expression,
> polymorphism, and screening.
> 17. Tools for comparing across different maize maps for QTL
> work involving different markers for different maps, and
> across species.
> 18. Bulk purchasing of reagents needed by the maize genetics community.
> How would you rate the priority of these needs, and any
> others that you may wish to add? Please reply to the maize
> net, maize at net.bio.net, where bulletin-board communications
> are appropriate.
> Do you have any suggestions as to solutions for these
> At future Maize Genetics Conferences, floor discussions will
> be carried out in greater depth, particularly as to how we
> might bring about the improvements that we all feel are
> needed. Thank you for your time and assistance.
> Ed Coe and Jeff Bennetzen for the ad hoc Maize Committee.
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