Antibodies for cytoplasmic background proteins

Tobias Baskin BaskinT at missouri.edu
Thu Feb 17 12:02:28 EST 2000


Greetings,

	Just a point. While actin is often used as a loading control
in RNA blots, it would be unlikely to be useful in microscopy because
actin is mostly filamentous in cells. You might like to consider a
stain for RNA which will label the cytoplasm smoothly and uniformly.

	Hope this helps,

			Tobias Baskin

At 8:28 PM +0000 2/2/00, Robert A. Bouchard wrote:
>Some colleagues and I are using confocal microscopy to investigate the
>locations of viral proteins in infected maize tissue and would like to
>locate a source of good antisera for one or two constituitve cytoplasmic
>proteins, such as actin or ubiquitin, to use as a control for general
>background.  Can anyone recommend a supplier of antisera know to work well
>with these or other constituitve maize proteins?  Thanks, RAB
>
>---
>
>Robert A. Bouchard, Affiliated Scholar
>Department of Chemistry
>561 University Street
>The College of Wooster
>Wooster, OH  44691
>phone:   (330) 263-2433
>Fax:     (330) 263-2386
>e-mail:  RBOUCHARD at ACS.wooster.edu
>
>
>---

        _      ____   ^      __   ____   Tobias I. Baskin
       /  \   /      / \    /   \ \            University of Missouri
      /   |  /      /   \   \      \              Biological Sciences
     /___/  /__    /___  \   \      \__            109 Tucker Hall
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