maize cDNA library

[tbutter at gwdg.de]

The Clontech-Kit for SMART-cDNA-Synthesis is the best I know. The 
Fast-Track I don't know - I think the best one is the QIAGEN 
RNeasy-Kit.

Good luck!
Thomas


At 2:33 PM +0000 11/29/01, Veronique Fontaine wrote:
>Good morning,
>
>I planned to build a cDNA library from 5 day's old maize tissue and
>tought to use
>
>FastTrack 2.0 kit from Invitrogen
>for mRNA isolation directly from the tissue
>and
>SMART cDNA library construction kit from Clontech
>to built the library
>
>What do you think of these 2 kits for maize? have you got any other
>good kit references for maize? Is it difficult to obtain mRNA from
>maize? Do you know the best method to do it?
>
>Thank you for your help
>
>Veronique Fontaine
>
>
>School of Biochemistry and Molecular Biology
>University of Leeds
>Leeds LS2 9JT,  UK
>Phone 44 113 233 1435
>Fax   44 113 233 3167
>email bmbvf at bmb.leeds.ac.uk