Postdoctoral Position

Asim Esen aevatan at vt.edu
Mon Dec 10 15:16:12 EST 2001


POSITION: A Postdoctoral Associate to determine the functions of the
Arabidopsis thaliana  beta-glucosidase and beta-galactosidase genes and/or
to investigate the mechanism of substrate specificity in
beta-O-glucosidases  and myrosinases (beta-S-glucosidases).

LOCATION: Department of Biology, Virginia Tech, Blacksburg, Virginia
USA 24061-0406.

DURATION: 2-3 years, starting January - February 2002.

RESPONSIBILITIES:
Construction, cloning and expression of beta-glucosidase and
beta-galactosidase cDNAs in bacteria, yeast or any other suitable host
system; purification and biochemical characterization of
beta-glucosidase and beta-galactosidase isozymes encoded by cloned cDNAs
with special emphasis on substrate specificity; and isolation and
characterization of the natural substrates hydrolyzed by each
isozyme. There will be also opportunity to learn homology modeling
and protein crystallization for 3-D structure determination.

QUALIFICATIONS:
The successful candidates for the position should have a strong
background and publications from previous work in molecular biology
or protein biochemistry.  Experience is necessary in standard
recombinant DNA techniques including cDNA synthesis, cloning and
expression, site-directed mutagenesis, and enzyme purification and
characterization.  Experience with plant systems is not necessary.
Candidates who have prior experience with protein expression in
yeast, E. coli and or baculovirus systems will be given preference.

CONTACT:  Send curriculum vitae and names of three individuals for
letters of recommendation to: Dr. A. Esen, Department of Biology,
Virginia Tech, Blacksburg, Virginia 24061-0406 USA.  Foreign
applicants may use e-mail (aevatan at vt.edu).  Tel: 540-231-5894; FAX:
540-231-9307.  Candidates may visit our lab website at:
http://www.biol.vt.edu/faculty/esen/glycosidaselab.





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