maize cDNA library

Veronique Fontaine bmbvf at bmb.leeds.ac.uk
Thu Nov 29 09:33:27 EST 2001


Good morning,

I planned to build a cDNA library from 5 day's old maize tissue and 
tought to use

FastTrack 2.0 kit from Invitrogen
for mRNA isolation directly from the tissue
and
SMART cDNA library construction kit from Clontech
to built the library

What do you think of these 2 kits for maize? have you got any other 
good kit references for maize? Is it difficult to obtain mRNA from 
maize? Do you know the best method to do it?

Thank you for your help

Veronique Fontaine


School of Biochemistry and Molecular Biology
University of Leeds
Leeds LS2 9JT,  UK
Phone 44 113 233 1435
Fax   44 113 233 3167
email bmbvf at bmb.leeds.ac.uk





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