DNA extraction from pollen grain

Guillemine, Pierre-Henri goun at iastate.edu
Thu Sep 19 09:15:54 EST 2002


Hi,

I am a French student working for the USDA-ARS in Ames, Iowa. I would like
to verify the presence of a transgene into pollen grain. My goal is simply
to make PCR analysis of the pollen I have collected. However, I can not
purify good quality of DNA for PCR. In fact, I have tried different standard
protocols for extraction of genomic DNA from polant tissue, But I have not
succeded in obtaining good DNA. I would like to know if any of you have
experienced a protocol suitable for pollen?
I would appreciate if you could give some tips...

Regards
Pierre-Henri Guillemine





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