[Maize] Call for maize gene suggestions for fluorescent protein tagging

David Jackson via maize%40net.bio.net (by jacksond At cshl.edu)
Thu Nov 30 10:29:44 EST 2006


Call for suggestions for generation of fluorescent protein tagged 
maize marker lines.

Through an NSF funded Plant Genome Award, we are making fluorescent 
protein tagged marker lines for cell, developmental and physiological 
studies in maize. Our goal in this project is to make fluorescent 
protein reporters for every known sub-cellular compartment in the 
plant. If you would like to suggest a gene for tagging please visit:

http://maize.tigr.org/tigr-scripts/maize/cellgenomics/gene_sub.pl

Please note that we are making fusions to genes controlled by their 
native regulatory sequences to avoid artifactual localization due to 
mis-expression. Other criteria that guide our selection of genes for 
tagging can be found on the website. In particular, details of how to 
check if your gene includes sufficient genomic sequence are available 
at the above site in the "FAQ" page. We will make decisions on 
currently tagged lines; we are especially interested in genes whose 
products are predicted to be in compartments not yet tagged (eg 
plasmodesmata, mitochondria, etc). Please check the website for the 
status of tagged genes already in the pipeline. Once you submit your 
gene sequence, we will first evaluate the gene based on the selection 
criteria on the website. We will notify you if and when your gene 
enters the tagging queue. We will then make the fluorescent protein 
tagged construct, transform it into maize and characterize the basic 
expression pattern. All tagged lines will be carried to the T2 
generation and seeds will be available to all members of the 
community through the Maize Genetics Stock Center.

Dave Jackson
Anne Sylvester
Agnes Chan




-- 
Dave Jackson,
Professor,
Cold Spring Harbor Laboratory,
1 Bungtown Rd.,
Cold Spring Harbor,
NY 11724   USA
tel 516 367 8467
fax 516 367 8369

website http://jacksonlab.cshl.edu


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