From medicago from net.bio.net  Wed Oct 11 11:20:14 2006
From: medicago from net.bio.net (medicago@net.bio.net)
Date: Wed Oct 11 11:27:24 2006
Subject: [Medicago] Request for RNA samples for FL-cDNA production
Message-ID: <9F9FBD4CF09A1446BE3EDFA1F3A49E870106853E@EXCHANGE.TIGR.ORG>

Dear Medicago (and Legume) Colleagues

 

As some of you may know, we were recently funded by USDA to generate
FL-cDNA sequences and clones from M. truncatula, L. japonicus and
Glycine max. The project, described in the attached summary, will
construct normalized libraries from several pools of RNA, end sequence
40,000+ clones from each species, re-array and fully sequence ~ 12,000
representative FL clones from each species. Both the sequences and
clones will be freely available, the sequences through the TIGR web site
and GenBank, the clones from the Noble Foundation in the USA and from
INRA-CNRGV, Toulouse in Europe.

While preparing the proposal, I had already solicited offers of RNA
donations from a number of you. Now that we are ready to get started, I
would like to ask any of you who have some RNA to spare if you would
consider providing some to go into one of the library pools. Ideally we
would like 5-10 ug of total RNA, but could get by with as little as 1-2
ug.

We are looking for RNA in the following categories, where the numbers
indicate the intended pool:

Flowers

1

Early seed

1

Late seed

1

Stems

1

 

 

Leaves

2

Biotic stressed leaves

2

Abiotic stressed leaves

2

Tissue culture

2

Elicitor-treated plants

2

 

Roots, early nodules

3

Roots, late nodules

3

Roots, biotic stress

3

Roots, abiotic stress

3

 

Within the biotic and abiotic stresses, we would be happy to have RNA
from you favorite stress(es) - the more the merrier as we try to
maximize the diversity of the libraries. Since one of the goals of the
project is to support genome annotation, there is a strong preference
for RNA samples from the varieties being sequenced (A18 for Medicago,
MG-20 for Lotus and Williams for soybean), but RNA from other accessions
may also be useful.

 

In order to get this project rolling, please send us some of your
samples ASAP. Shipping at room temperature under ethanol should be fine.
Dry ice if you prefer. We can provide a FedEx/DHL account number if you
need it. If you are shipping from abroad, please contact me first so
that I can send you a Toxic Substances Control Act (TSCA) certification
to include with your package and speed it through customs.

 

Thank you for your support. I look forward to your donations.

 

Best wishes

 

Chris

 

______________________________

Chris Town

Associate Investigator

The Institute for Genomic Research

9712 Medical Center Drive

Rockville, MD 20850

Office Phone: 301-795-7523

To page me at TIGR: 301-795-7000

Fax: 301-838-0208

Home Phone: 301-990-0878

Cell Phone: 301-204-6300