[Medicago] ENOD12B promoter

medicago from net.bio.net via medicago%40net.bio.net (by medicago from net.bio.net)
Fri Feb 22 15:38:44 EST 2008


Dear Benedikt,

I suspect that the promoter you are referring to might be from the 
alfalfa MsENOD12B gene, or perhaps even pea PsENOD12B. In M. truncatula 
there is only one ENOD12 gene, so no need for A & B labeling. What is 
certain is that, whatever the plant, the promoter is *not* root hair 
specific, although it is expressed in root hairs, and is induced by NFs 
and during rhizobial infection/nodulation.

I hope this information is helpful to you.

Best regards,

David Barker






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>    1. Promotor ENOD12B from Medicago truncatula (medicago from net.bio.net)
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> Message: 1
> Date: Fri, 22 Feb 2008 10:16:33 +0100 (CET)
> From: medicago from net.bio.net
> Subject: [Medicago] Promotor ENOD12B from Medicago truncatula
> To: medicago from net.bio.net
> Message-ID: <1644.129.132.136.169.1203671793.squirrel from www.vis.ethz.ch>
> Content-Type: text/plain;charset=iso-8859-1
>
> Hallo everybody,
>
> I am looking for the genomic sequence of the ENOD12B (Early Nodulin 12B)
> Promotor originating from Medicago truncatula. It should be a roothair
> specific promotor. Currently I am sequencing an old plasmid  from our lab,
> which should contain this promotor. Unfortunately I cannot verify the
> sequence in the plasmid to be the supposed ENOD12B Promotor. A nblast in
> the NCBI-database of my sequenced DNA fragment gives me a hint, that my
> sequence comes from medicago, but the Sequence alignment is more or less
> bad.
>
> I hope you understand my problem. Unfortunately in there is more data
> aviable about the construct I am sequencing (very old one, and the person
> who made it already left the lab). I will be very greatful, if there is
> anybody who could help me.
>
> Best wishes,
>
> Benedikt
>
>
>
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> End of Medicago Digest, Vol 18, Issue 2
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-- 

David G. Barker,
Laboratory of Plant-Microbe Interactions (ex LBMRPM),
UMR INRA/CNRS (441/2594),
Chemin de Borde-Rouge, Auzeville,
BP 52627,
31326 CASTANET-TOLOSAN Cedex,
FRANCE
Tel (33).5.61.28.54.15
Fax (33).5.61.28.50.61
David.Barker from toulouse.inra.fr
 
http://www.toulouse.inra.fr/centre/lipm/index.htm



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