From medicago from net.bio.net  Wed Mar  4 12:12:01 2009
From: medicago from net.bio.net (medicago@net.bio.net)
Date: Wed Mar  4 12:21:23 2009
Subject: [Medicago] Newsletter CNRGV - March 2009 - French Plant Genomic
	Resource Center
Message-ID: <20090304171201.7714F2F510@merlin.cnrgv.toulouse.inra.fr>

If you can see this HTML message correctly, click here to view in your web browser : http://cnrgv.toulouse.inra.fr/communication/gene_home_newsletter_march_2009
From medicago from net.bio.net  Mon Mar 23 16:47:19 2009
From: medicago from net.bio.net (medicago@net.bio.net)
Date: Mon Mar 23 16:47:30 2009
Subject: [Medicago] chloroplast-free DNA
Message-ID: <9F9FBD4CF09A1446BE3EDFA1F3A49E8702971C37@EXCHANGE.TIGR.ORG>

Hi

 

I'm trying to isolate a lot of CP-free DNA for sequencing without going
to the trouble of nuclear isolation.

I've germinated seed in the dark and collected hypocotyls (about 6g).
However, based upon a similar approach with Brassica, I don't think I'll
get enough DNA. I suspect that compared with leaves, the hypocotyls have
big cells and thus fewer nuclei per gram

 

Any suggestions to get increased CP-free biomass?

 

Thanks

 

Chris

 

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Chris Town

Professor

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