(none)

[MBY134 at sysh.surrey.ac.uk]

Does anyone have a good method for extracting good yields of DNA from
a 50Kb recombinant cosmid clone - in pHC79 - a ColE1 based lambda cosmid.
Alkaline extraction of plasmid DNA gives very low yields - too low to
band on caesium chloride. Any ideas?

Johnjoe McFadden
Department of Microbiology
University of Surrey
Guildford, Surrey, UK.

       'Ever tried? Ever failed? No matter. Try again. Fail again.
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