MBY134 at sysh.surrey.ac.uk MBY134 at sysh.surrey.ac.uk
Fri Aug 10 06:01:40 EST 1990

Does anyone have a good method for extracting good yields of DNA from
a 50Kb recombinant cosmid clone - in pHC79 - a ColE1 based lambda cosmid.
Alkaline extraction of plasmid DNA gives very low yields - too low to
band on caesium chloride. Any ideas?

Johnjoe McFadden
Department of Microbiology
University of Surrey
Guildford, Surrey, UK.

       'Ever tried? Ever failed? No matter. Try again. Fail again.
        Fail better!
         ACCCCCCCCCCC'BDDDDD           Samual Becket

More information about the Methods mailing list