visualizing small pcr fragments

aschulman at cc.helsinki.fi aschulman at cc.helsinki.fi
Mon Feb 12 12:55:13 EST 1990


In article <136 at uncmed.med.unc.edu>, danielg at uncmed.med.unc.edu writes:
> I have a small technical problem, perhaps one of you can give me a simple
> solution.
> 
> When I electrophorese my pcr products, I am looking for a band around 
> 200 bases long (pretty small).  The problem is that the band usually comes
> out right at the dye front, which makes UV visualization a slight problem.
> 
> Is there another dye I can use that will run a bit faster or slower than
> bromphenol blue?  I could try just using glycerol and edta in my loading
> buffer, leaving out the dye.
> 
> All suggestions welcome...
> 
> Daniel G. Sinclair
> Rsch Tech II
> University of NC, Chapel Hill
> 
>  Disclaimer: My opinion means | 'If you only knew how much I was holding
>              nothing, but His |  back, you would commend me'
>              means everything.|      - Charles Spurgeon, 19th century
>                               |        evangelist (on humor in preaching)





You can use xylene cyanol (Sigma X0377 and many other suppliers) which runs
more slowly than bromophenol at about .05% final concentration.

ASCHULMAN at FINUH.BITNET or ASCHULMAN at CC.HELSINKI.FI



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