PCR * Enzymes cut ss-DNA?

HARTLAUB at dulruu51.bitnet HARTLAUB at dulruu51.bitnet
Sat Feb 3 13:25:00 EST 1990


Can anybody help me ?

Does anybody know whether larger fragments than the usual 20- to 30-mers
of oligonucleotides can be used as primers for the PCR ?
  (My aim is to connect a certain marker-gene (ca. 1500 bp) instead of a
   restriction-site directly to the PCR-fragments to avoid cloning.)

Which restriction enzymes do cut single-stranded DNA ? (HindIII works).



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