PCR * Enzymes cut ss-DNA?
HARTLAUB at dulruu51.bitnet
HARTLAUB at dulruu51.bitnet
Sat Feb 3 13:25:00 EST 1990
Can anybody help me ?
Does anybody know whether larger fragments than the usual 20- to 30-mers
of oligonucleotides can be used as primers for the PCR ?
(My aim is to connect a certain marker-gene (ca. 1500 bp) instead of a
restriction-site directly to the PCR-fragments to avoid cloning.)
Which restriction enzymes do cut single-stranded DNA ? (HindIII works).
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