phenol-chloroform alternatives

ann baker biology dept 303 491 5307 aebaker at csugreen.bitnet
Wed Feb 14 11:41:45 EST 1990


sambrook et al mention guanadine+ethanol; formamide+dialysis
as alternatives to phenol-chloroform-isoamylalcohol for
removing protein and fat from dna. if anyone has run parallel
samples phenol-chlo versus guanidine ; phenol-chlor versus
formamide, i would be interested to know:
    differences in yield of DNA
    disadvantages of these other methods

a reply to an earlier request stated that oncor has a kit
using saturated NaCl for cleaning DNA and the yields were
the same. oncor 301 963 3500.

ann eileen miller baker
biology department
colorado state university
fort collins 80523
303 491 5307
aebaker at cusgreen



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