visualizing small pcr fragments

danielg at uncmed.med.unc.edu danielg at uncmed.med.unc.edu
Wed Jan 31 12:13:48 EST 1990


I have a small technical problem, perhaps one of you can give me a simple
solution.

When I electrophorese my pcr products, I am looking for a band around 
200 bases long (pretty small).  The problem is that the band usually comes
out right at the dye front, which makes UV visualization a slight problem.

Is there another dye I can use that will run a bit faster or slower than
bromphenol blue?  I could try just using glycerol and edta in my loading
buffer, leaving out the dye.

All suggestions welcome...

Daniel G. Sinclair
Rsch Tech II
University of NC, Chapel Hill

 Disclaimer: My opinion means | 'If you only knew how much I was holding
             nothing, but His |  back, you would commend me'
             means everything.|      - Charles Spurgeon, 19th century
                              |        evangelist (on humor in preaching)



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