geoffk at usage.csd.unsw.oz.au
Fri Jul 13 03:28:41 EST 1990
We are presently using Cu/phenanthroline to footprint the promoter of the
yeast LPD1 gene but have come across a region which does not give a good
ladder in the absence of protein. As luck would have it, this region
is a transcriptional activator binding site (we know that from other work).
We would like to use the MPE-Fe footprinting system of Hertzberg & Dervan
to gte around this (and to give us a more precise picture of what is
happening at some other sites) but there are a few problems:
1) Is there a commercial distributer of MPE-Fe? I have been
unable to find any here.
2) Have those out there who have used this method (& I hope
that there are some) had to synthesise your own (if there
is no commercial source) or were you able to get it from
3) I am willing to synthesise it if necessary, but the Australian
branch of May & Baker does not have a listing for
p-carboxymethidium chloride (5-methyl-6-(4-carboxylphenyl)-
3,8-diaminophenanthridinium chloride). [ the papers cite
them as the source ]. Is ther an alternative source?
I would appreciate it if anyone who has used this technique could help.
School of Biochemistry
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