Bacteria and PCR

Jaime Arenas arenas at IAGO.CALTECH.EDU
Tue Jul 31 18:41:30 EST 1990


  
  
  I'm trying to find the conditions under which the insert in a plasmid
  can be amplified by adding the bacteria bearing the plasmid directly
  to a PCR reaction using microtitter plates and a BIOS thermal cycler
  oven.
  
  I've been successfull doing PCR with bacteria in an Ericomb machine.
  However, there is a limited number of sample wells in the block.
  
  Has anybody out there determined the conditions for PCR with bacteria
  in the BIOS oven using microtitter plates?
  
  Any tips will be appreciated.
  


Jaime Arenas                              There is no places,
Division of Biology 147-75                there is beginnings and ends,
CALTECH                                   but nothing is ever forgotten.
Pasadena, CA 91125

ARENAS at CITIAGO.BITNET
ARENAS at IAGO.CALTECH.EDU                   (NO, there are no misspellings)




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