Bacteria and PCR
arenas at IAGO.CALTECH.EDU
Tue Jul 31 18:41:30 EST 1990
I'm trying to find the conditions under which the insert in a plasmid
can be amplified by adding the bacteria bearing the plasmid directly
to a PCR reaction using microtitter plates and a BIOS thermal cycler
I've been successfull doing PCR with bacteria in an Ericomb machine.
However, there is a limited number of sample wells in the block.
Has anybody out there determined the conditions for PCR with bacteria
in the BIOS oven using microtitter plates?
Any tips will be appreciated.
Jaime Arenas There is no places,
Division of Biology 147-75 there is beginnings and ends,
CALTECH but nothing is ever forgotten.
Pasadena, CA 91125
ARENAS at CITIAGO.BITNET
ARENAS at IAGO.CALTECH.EDU (NO, there are no misspellings)
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