KING-SIZE-CLONING

fred FRED at blekul13.bitnet
Thu Jun 28 04:46:34 EST 1990


Admitting the superiority of the people listed below is not easy.
But then again we never really believed a world record was in the making
here. I do appreciate the time you took to respond. Maybe we can build
a "usefull-thing-to-know-but-kept-silent-for-unknown-reasons"-kind
of mailbox here.
List of responses:
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Date: Wed, 27 Jun 90 15:33:31 -0700
From: "Marc D. Perry" <mdperry at boulder.Colorado.EDU>
Subject: Re: king-size-cloning
To: FRED at BLEKUL13.BITNET
Organization: MCD Biology, University of Colorado, Boulder

Fred,

Many of the early ColE1/pMB9 derived vectors (from which pUC's are
derived) were 10 kb, and genomic libraries constructed in them had
avg. insert sizes of 10 kb (10 + 10 = 20).  So I wouldn't call
Guinness just yet.

Marc P.
---------------------------------------------------------
Marc D. Perry                               (303) 492 8258
MCD Biology, Univ. of Colorado, Boulder, CO 80309-0347
internet: mdperry at boulder.colorado.edu
--
Marc D. Perry                               (303) 492 8258
MCD Biology, Univ. of Colorado, Boulder, CO 80309-0347
internet: mdperry at boulder.colorado.edu
=========================================================================
Date: Wed, 27 Jun 90 10:52 EDT
From: Department of Molecular Karma <YABLONSKY at MBCL.BITNET>
Subject: Re: KING-SIZE-CLONING
To: FRED at BLEKUL13.BITNET

Minor league....but keep trying!!!
=========================================================================
Date: Tue, 26 Jun 90 13:25 EST
From: SCOLNIPA%ESVAX at dupont.com
Subject: RE: king-size-cloning
To: FRED at blekul13.BITNET
X-Vms-To: ESPRNT::IN%"FRED%blekul13.bitnet at genbank.bio.NET"

No way.  We have several plant clones in pUC that are 15 kb or bigger.
=========================================================================
Date: Tue, 26 Jun 90 10:58:21 EST
To: fred at blekul13.bitnet
Subject: king-size-cloning

I was not too surprised.  I had cloned several 10 to 16 kb inserts in
pUC plasmids.  In addition cosmids (derivatives of pUC plasmids with cos
site) take up 40 kb inserts and stably replicate as plasmids.
K. Bhat
=========================================================================
Date:     Tue, 26 JUN 90 17:39:05 GMT
From:     PHILLIPSA@ LARS.AFRC.AC.UK
To:       METHODS@ DLGM.DARESBURY.AC.UK

Re: KING-SIZE-CLONING

Fred,

My record is a 21.9 kbp fragment of tomato chloroplast
DNA in pUC8 (A. Phillips, Curr. Genet (1985) 10:147-152.
Raise you 13kbp?

Andy
=========================================================================
Date: Tue, 26 Jun 90 07:14:18 EDT
To: FRED at blekul13.BITNET
Subject: Re:  king-size-cloning

Fred,

I don't find subcloning a 9 kb fragment into pUC unusual.  We have inserted
8 and 10 kb fragments before.

douglas prasher
woods hole oceanographic inst
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FRED at BLEKUL13.
Acknowledge-To: <FRED at BLEKUL13>



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