restriction

FRED at blekul13.bitnet FRED at blekul13.bitnet
Tue Mar 6 03:54:34 EST 1990


Relevant to the problem of restriction sites in close proximity is
rstriction of short DNA sequences. An interesting piece was published
in the NEB transcript December 1989 (New England Biolabs). They have checked
oligos of 8,10,12 or 14 bases with all the most commom enzymes. One example:

Enzyme    Oligo         Length

   BamH I   c ggatcc g        8      10% cleavage after 2 hrs
            cg ggatcc cg     10     >90%
            cgc ggatcc gcg   12     >90%

Might be usefull.

Fred Van Leuven  Human Genetics Leuven Belgium
FRED at BLEKUL13
Acknowledge-To: <FRED at BLEKUL13>



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