about Spl I

[Francis Ouellette]

In a recent message Johan de Boer (FS300512 at YUSOL) asks:

>I am trying to find a source for the following restriction
>enzyme:
>
>       SplI or PfuI or PspI
>
>They are isoschizomers.  If anyone knows of a company that
>sells one of these, would you please let me know?


If I look up in PC/Gene using the REDIT program (the
restriction enzyme editor) I find the following info:

>
>RESTRICTION ENZYME DATA EDITOR.
>
>Enzyme name      
Spl I____Y
>Recognition site 
CGTACG___________Y
>Cut offset: 1__ bases.
>
>Isoschizomers.
>
__________________________________Y
>Availability.
>
Amersham._________________________Y
>

The (old) Amersham catalogue I have (1989) claims that
Amersham is the only distributor of Spl I.  But PC/Gene
doesn't seem to know that Spl I has any isoschizomers ...
But the REDIT program of PC/Gene is a bit out of date ...
Maybe the next update ;-)


disclaimer:  I am in no way, shape or form an employee
or a sales rep for IntelliGenetics or Amersham.

"And that's the truth Ruth"
                            (Do the right thing, Spike Lee)



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"Je cherche a comprendre"       Jacques Monod

B.F. Francis Ouellette  Bitnet (Netnorth): userBFFO at SFU
                      Internet: userBFFO at cc.sfu.ca
                                Ouellett at whistler.sfu.ca
address: Dept of Biol. Sci., Simon Fraser University
         Burnaby, BC, V5A 1S6, Canada, (604)291-3021
                                  FAX: (604)291-3496

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PS.  I realize that being from Canada it may be politically
unwise (and this be the wrong place for it) to have a
signature "en francais" but hey, pourquoi pas!

;-)