PCR cloning
Chris_Jones at vme.ccc.nottingham.ac.uk
Chris_Jones at vme.ccc.nottingham.ac.uk
Fri Nov 2 10:54:07 EST 1990
I've just seen an ad for Invitrogens PCR cloning kit where they
claim that PCR products always have a 3' A added on. Does anyone
know anything about this (reference?). I have always done a klenow step
before cloning but didn't realise that the ends were ragged because extra
bases were added on? Quite a few of my applications would be wrecked by an
extra A so any tips about eliminating them (if they exist) would be great.
Chris Jones
Biochemistry Dept
Nottingham Univ.
U.K.
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