MBY134 at sysh.surrey.ac.uk MBY134 at sysh.surrey.ac.uk
Tue Oct 23 07:53:55 EST 1990

Does anybody have any particular tips for doing inverse-PCR?
We've tried alterring magnesium conc., dilute ligations to encourage
intramolecular ligation, long extension times - but no product (dispite
direct PCR working on template)
Any ideas welcome.

           "Ever tried? Ever failed?. No matter
            Try again. Fail again. Fail better!
                             Samual Beckett

Johnjoe McFadden
University of Suttey, UkK

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