asymmetric PCR + sequencing

WALLEN at CSC.FI WALLEN at CSC.FI
Tue Oct 23 12:59:00 EST 1990


I am planning to use asymmetric PCR (with chicken chromosomal DNA as a
template) + direct sequencing of the amplification products (preferably
with Sequenase) to get some sequence of a gene which is difficult to
clone.  I have some experience of sequencing PCR products, but not of
this particular application.  I would like to hear about protocols which
other people have found out to work well.  Any hints, suggestions etc.
would be appreciated.

Thank you in advance.

Mika Wallen
Univ. Tampere
Dept. Biomed. Sci.
P.O.Box 607
33101 Tampere
Finland 



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