Southerns with seiving agarose

[Jim Gerlach]

We are doing some southern blots of PCR'd cDNA and have been separating a
650bp DNA fragment using a gel consisting of 2.75% wide-range agarose (Sigma)
and 1.0% standard agarose (Pharmacia). We seem to be getting poor capillary
transfers to nitrocellulose and wonder if this mix may be contibuting. Has
anyone had experience with transfers from this or similar agarose mixes?

Thanx in advance for any suggestions, comments, etc.

Jim

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James.H.Gerlach at QueensU.CA                    Queen's University
GERLACHJ at QUCDN.QueensU.CA (NetNorth/BITNET)   Room 358, Botterell Hall
tel (613) 545-6446                            Kingston, Ontario
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