Genomic substraction

[Yves Bertheau]

We have some troubles during the course of a genomic substraction with ligation

Does somebody try ligation of oligonucleotides adaptators (one phosphorylated at
PCR using one strand of the adaptataors as a primer ?

We were trying to use Ausubel's protocol...

Any help would be appreciated.

Thanks in advance.

Yves Bertheau, Pathologie vegetale, INRA, PAris France.