Are you decontaminating your ethidium bromide solutions?
num208jn at MBDS.NRC.CA
Wed Aug 14 08:28:12 EST 1991
In article <1991Aug14.024044.20602 at metro.ucc.su.OZ.AU>, weiss at ee.su.oz.au writes:
>This one surprised us. We've been decontaminating our waste ethidium
>bromide stocks with hypochlorite, and now discover that it's *not*
>recommended. The Sambrook et al Molecular Cloning Lab Manual (2nd Edn)
>says on pages 6.16 - 6.17 that you shouldn't use bleach, but rather should
>go through a choice of filtration methods, including the use of Amberlite
>XAD-16 or powdered activated charcoal (for >0.5 ug/ml eth br)!!!!!!!!
>Does anyone have any better suggestions??
>weiss at ee.su.oz.au
>"usual disclaimers apply"
There is a paper [Ethidium Bromide: Destruction and Decontamination of
Solutions. Lunn, G. and Sansone. E.B. Analytical Biocehmistry
162:453-458, 1987] which describes various ways to destroy ethidium
bromide and looks at the mutagenicity of the various products of the
different destruction methods. It's probably worth a bit of a look.
Unfortunately, all of the methods which "work" are tedious.
Hope this helps.
John Nash, Internet: num208jn at mbds.nrc.ca
Institute for Biological Sciences,
National Research Council of Canada, Ottawa, Canada K1A 0R6.
Disclaimer: All opinions are mine, not NRC's!
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