ss DNA cleavage by restriction ennzymes

Bruce Roe BROE at AARDVARK.UCS.UOKNOR.EDU
Wed Aug 7 08:46:00 EST 1991


Rich Roberts brought out the major reason why we never published
these experiments, i.e. if the lack of ss cleavage activity at elevated
temperatures was due to a factor other than melting of a small ds
DNA region in the ss DNA template.  Maybe he could post the list of
relevant references and someone could come up with a more definative
answer to this unresolved issue.
--bruce roe



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