Pfu Polymerase?

John Altman altman at cmgm.stanford.edu
Mon Dec 30 21:53:15 EST 1991


I've been having mixed results running PCR reactions with Stratagene's
Pfu polymerase (3 of 4 templates amplify with Pfu -- all 4 amplify
with Taq).  I've tried reducing annealing temperatures, adding glycerol
and DMSO (Stratagene's recommendations), etc.  Has anyone out there had
similar experiences with Pfu, or better luck?

			Thanks
			John
-- 
John Altman				altman at cmgm.stanford.edu
HHMI					(415) 723-7218 
Beckman Center B-219
Stanford University
Stanford, CA 94305-5428
--
John Altman				altman at cmgm.stanford.edu
HHMI					(415) 723-7218 
Beckman Center B-219
Stanford University



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