rcbrown at casbah.acns.nwu.edu
Thu Dec 19 15:11:44 EST 1991
Hello mobio jocks,
I got a little problem here with in-situ hybridization. I am currently using
S-35 RNA probes. I have gotten the procedure to work with chicken brains.
However, pituitary seems to be another matter. Sense control probes gave a
signal that was comparable to antisense probes. I am interested in conditions
that would relate to pituitary or other tissues. Specifically, cpms/ml, probe
size, specific activity of the probes, stringency of the washes (do you use
mercaptoethanol in your washes), temperature of washes, how long you can store
tissue in OTC at -70 degrees. I really need this procedure to work because I
am using it to screen PCR clones. I would appreciate any help I can get.
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