Subtractive PCR?

Clifford J Beall cbeall at magnus.acs.ohio-state.edu
Wed Dec 18 16:33:47 EST 1991


Dear Methods people,

Does anyone have a method for subtracting cDNA prior to PCR?
We are using degenerate primers and want to clone a cell specific
message.  It seems one could hybridize with [+] cDNA with [-] RNA,
remove hybrids, then treat the remainder with RNAse.  Is HAP still the
method of choice for hybrid removal and does anyone know conditions
for this?  Thanks in advance.
				Cliff Beall
				Ohio State Biotech. Ctr.



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