glycogen carrier for nucleic acid ppt

Cheung C. Yue ccy at po.CWRU.Edu
Wed Dec 18 18:29:50 EST 1991


I am interested in using glycogen as a carrier for nucleic acids
ppt.  Tracy (Prep Biochem 11:251, '81) described purification of
glycogen for such use.  Steps include micrococcal nuclease, proteinase
K, then extensive phenol extraction.  Another paper I've seen 
said they just took glycogen from Sigma, dissolved it in water and
use.  Does anyone have any experience with this problem?  Are there
problems with the second approach?

ccy at po.cwru.edu
  
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